![]() In addition to its neurotrophic activity on the nervous system and retina, PEDF was recently found to be a strong inhibitor of angiogenesis in the eye and it might be responsible for maintenance of the avascular status of corneal tissue ( 6). Pigment epithelium-derived factor PEDF 50-kDa glycoprotein initially isolated from retinal pigment epithelial cells, is initially identified as neuronal differentiation factor produced by cultured human retinal pigment epithelial cells ( 5). Study indicates that angiogenesis correlates with disease stage and metastasis in prostate cancer ( 2), and occurs in those prostatic intraepithelial neoplasms ( 3) and latent carcinomas ( 4) with the potential for progression to invasive disease. So far, it is no doubt that primary tumor and metastatic growth must be dependent on neovasculature formation ( 1). The incidence and mortality of prostate cancer is mainly rest with invasion and metastasis of primary tumor. Prostate cancer is a leading cause of morbidity and mortality among males. PEDF-mediated inhibition of prostate cancer growth and metastases could thus have a major impact on existing therapies for prostate cancer. Furthermore, PEDF overexpression also greatly inhibited tube formation in vitro, and decreased production of VEGF in DU145 cells.Ĭonclusions: It was suggested that the effects of PEDF on primary tumor growth and lung metastasis appear associated with inhibition of angiogenic tumor response. Compared to control, MVD decreased significantly in the mice transfected with PEDF. Results: The growth of implanted tumor was significantly reduced in sizes, and the lung metastases were also completely inhibited. Human microvessel endothelial cells (HMVEC) tube formation was assayed in vitro. Intratumoral microvessel density (MVD) was detected by immunohistochemistry using mouse anti-human CD31 monoclonal antibody. The tumor volume (mm 3 ) was measured by applying the formula for approximating the volume of a spheroid, and lung metastases are evaluated using India ink staining. In vivo, the prostate cancer cells DU145 with overexpression of PEDF were injected s.c. In this study, we examined the consequences of overexpression of pigment epithelium-derived factor (PEDF) on both prostate cancer primary tumor growth and metastasis development. Background: Human prostate mortality is associated with tumor invasion and metastasis. ![]()
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